ABSTRACT
Summary Anamnesis: eight Silla Argentina breed horses imported from Argentina presented with an abnormal gait and skin lesions. Clinical and laboratory findings: all cases showed different degrees of hind limb hyperflexion, atrophy of the lateral digital muscles, skin lesions, and poor body condition. They underwent several clinical and laboratory examinations, including endoscopy of the upper respiratory tract, skin and liver biopsy, blood count, biochemical profile, and electromyography that showed evidence of a neuropathy and dermatitis. Treatment approach: the horses were treated with phenylbutazone and thiamine. The response was patient dependent with different degrees of recovery resulting. Conclusion: this is the first report of an outbreak of bilateral Australian Stringhalt in Colombia and the first associated to skin lesions according to the veterinary medicine scientific literature.
Resumen Anamnesis: ocho caballos de raza silla Argentina importados de Argentina presentaron un andar anormal y lesiones de piel. Hallazgos clínicos y de laboratorio: todos los animales presentaron hiperflexión de los miembros anteriores de forma bilateral, en diferentes grados de severidad; atrofia del músculo digital lateral, lesiones de piel y condición corporal deficiente. Se realizaron endoscopias, biopsias de piel, hematología, química sanguínea, electromiografías que indicaron una neuropatía y dermatitis. Abordaje terapéutico: los caballos fueron tratados con fenilbutazona y tiamina, con una respuesta variable entre los animales. Conclusión: este es el primer reporte de un brote de arpeo australiano en Colombia y el primero asociado a lesiones de piel de acuerdo a la literatura científica médico-veterinaria.
Resumo Anamnese: oito cavalos de raça Silla Argentina foram importados a partir da Argentina por una marcha anormal e lesões cutâneas. Achados clínicos e de laboratório: todos os animais apresentaram hiperflexão bilateral dos membros anteriores com diferentes graus de gravidade; atrofia do músculo digital lateral, lesões de pele e condição corporal baixa. Foram efectuadas endoscopias, biópsias de pele, hematologia química sanguínea e electromiografias que indicaram uma neuropatia e uma dermatitis. Abordagem terapêutica: os cavalos foram tratados com fenilbutazona e tiamina obtendo uma resposta variável entre os animais. Conclusão: este é o primeiro relatório de arpejo Australiano reportado na Colômbia e o primeiro associado com lesões de pele de acordo com a literatura científica médico-veterinária.
ABSTRACT
Taraxacum officinale F. H. Wigg, Asteraceae, is frequently misidentified or substituted with Hypochaeris radicata L., Asteraceae (false dandelion). To increase our knowledge of T. officinale and differentiate it from H. radicata, we investigated the two species using a combination of taxonomy, microscopy, and chromatographic studies via fingerprint profiles. Micromorphological characteristics were studied using scanning electron microscopy, while optic light microscopy was used for histochemical observations. Fingerprint profiles were constructed using HPTLC. T. officinale was found to have a morphologically distinct type of pluricellular trichomes that can be used to differentiate the two species, as these structures were not identified in H. radicata samples. Furthermore, two types of laticiferous vessels may also be distinctive characteristics of T. officinale at species level. In addition, the HPTLC data derived from methanolic extracts of H. radicata and T. officinale roots showed clearly different chemical profiles. Thus this study establishes the authenticity of T. officinale, and the observed parameters could help minimize drug substitutions in herbal medicines.
ABSTRACT
<p><b>OBJECTIVE</b>To analyse qualitative and quantitative phytochemical and evaluate in vitro antioxidant properties of various alcoholic and aqueous extracts of leaf and root parts of Hypochaeris radicata.</p><p><b>METHODS</b>Preliminary phytochemical analysis for alkaloids, cardiac glycosides, flavonoids, glycosides, phenols, resins, saponins, steroids, tannins, terpenoids and triterpenoids and quantitative phytochemical analysis for alkaloids, total phenolics, total flavonoids, tannins, saponins and ascorbic acid were made by following standard procedures. In vitro antioxidant properties were evaluated by assessing DPPH(•), NO(•) and ABTS(•+), radical scavenging abilities and assaying the reducing power, β-carotene and antihemolytic activities by adapting standard methods.</p><p><b>RESULTS</b>The quantitative phytochemical analysis of this species exhibited the presence of alkaloids, total phenolics, total flavonoids, tannins, saponins and ascorbic acid in considerable quantity. The in vitro antioxidant activity of the species, Hypochaeris radicata clearly demonstrated that both the leaf and root parts have prominent antioxidant properties.</p><p><b>CONCLUSIONS</b>From this study, it can be concluded that the species is effective in scavenging free radicals and has the potential to be a powerful antioxidant.</p>
ABSTRACT
Objective: To analyse qualitative and quantitative phytochemical and evaluate in vitro antioxidant properties of various alcoholic and aqueous extracts of leaf and root parts of Hypochaeris radicata.Methods:glycosides, phenols, resins, saponins, steroids, tannins, terpenoids and triterpenoids and quantitative phytochemical analysis for alkaloids, total phenolics, total flavonoids, tannins, saponins and ascorbic acid were made by following standard procedures. In vitro antioxidant properties were evaluated by assessing DPPH Preliminary phytochemical analysis for alkaloids, cardiac glycosides, flavonoids,?, NO? and ABTS?+, radical scavenging abilities and assaying the reducing power, β-carotene and antihemolytic activities by adapting standard methods.Results:alkaloids, total phenolics, total flavonoids, tannins, saponins and ascorbic acid in considerable quantity. The in vitro antioxidant activity of the species, Hypochaeris radicata clearly demonstrated that both the leaf and root parts have prominent antioxidant properties.Conclusions:From this study, it can be concluded that the species is effective in scavenging free The quantitative phytochemical analysis of this species exhibited the presence of radicals and has the potential to be a powerful antioxidant.
ABSTRACT
The aim of present study was to estimate the total phenolic and flavonoid contents and to investigate in vitro antioxidant potential of methanolic leaf and root extracts of the herb, Hypochaeris radicata L. (Asteraceae). Antioxidant activity was assessed by using 2,2- diphenyl-1-picryl-hydrazyl (DPPH•) assay, reducing power activity, [2,2’-azino-bis(3- ethylbenzthiazoline-6-sulphonic acid)] ABTS•+ assay and ferrous ion chelating activity. Here, butylated hydroxytoluene (BHT), ascorbic acid (ASA), trolox and EDTA were used as standard antioxidants. The total phenolic and flavonoid contents were also determined and expressed in gallic acid and quercetin equivalent respectively. The results of the study indicate that the methanolic extracts of the leaf and root of H. radicata posses significant scavenging activity against DPPH• (97.99% for leaf and 96.44% for root at 250μg/ml each) and ferrous ions chelating activity (38.69% for leaf and 40.52% for root at 5000μg/ml each), reducing power activity (1.38 absorbance at 600μg/ml for leaf, 0.45 absorbance at 700 μg/ml for root) and free radical scavenging activity (ABTS•+) (2706.73 for leaf and 2028.37μmol for root TE/g). The free radical scavenging and antioxidant activities may be attributed to the presence of adequate phenolic (gallic acid content is 125.5μg/10mg in leaf and 133.06μg/10mg in root) and flavonoid compounds (105.76μg/2mg in leaf and 55.16μg/2mg in root). This study revealed that the methanolic extracts of both leaf and root of H. radicata has demonstrated significant antioxidant activity.